Neb noti hf pdf#

Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Priority to US95920307P priority Critical Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc Priority to EP08796186A priority patent/EP2167655B1/en Publication of EP2468854A1 publication Critical patent/EP2468854A1/en Application granted granted Critical Publication of EP2468854B1 publication Critical patent/EP2468854B1/en Status Active legal-status Critical Current Anticipated expiration legal-status Critical Links

Original Assignee New England Biolabs Inc Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) The purified PCR products was digested in a 30 l reaction consisting of 0.5 l of NotI HF (NEB), 0.5 l of PacI (NEB), 3 l of Cut Smart Buffer (NEB). Enter primer sequences (with up to 3 ambiguous bases). If needed, modify the recommended primer concentration. Select the polymerase or kit from the list of products. Select the product group of the polymerase or kit you plan to use. ( en Inventor Zhenyu Zhu Aine Blanchard Shuang-Yong Xu Shengxi Guan Hua Wei Penghua Zhang Dapeng Sun Siu-Hong Chan Current Assignee (The listed assignees may be inaccurate. Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Granted Application number EP11075111A Other languages German ( de)

Neb noti hf pdf#

Google Patents High fidelity restriction endonucleasesĭownload PDF Info Publication number EP2468854A1 EP2468854A1 EP11075111A EP11075111A EP2468854A1 EP 2468854 A1 EP2468854 A1 EP 2468854A1 EP 11075111 A EP11075111 A EP 11075111A EP 11075111 A EP11075111 A EP 11075111A EP 2468854 A1 EP2468854 A1 EP 2468854A1 Authority EP European Patent Office Prior art keywords cleavage restriction endonuclease buffer mutation activity Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents EP2468854A1 - High fidelity restriction endonucleases All HF-restriction enzymes come with Gel Loading Dye, Purple (6X). EP2468854A1 - High fidelity restriction endonucleases High-Fidelity (HF ) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer ( rCutSmart Buffer ).